ABSTRACT
OBJECTIVE:To optimize the water extraction technology of Yinju jiedu oral liquid,and provide reference for the industrial production of the preparation. METHODS:According to the investigation of extraction time-extraction rate curves of chlo-rohenic acid of Yinju jiedu formula and extraction rate of chlorohenic acid in Lonicera japonica and other combined medicinal mate-rials in the formula,decoction methods and time of L. japonica were determined. Using the comprehensive scores of linarin,harpa-goside,(R,S)-epigoitrin,psoralen+angelicin contents and dry extraction yield as indexes,L9(34)orthogonal test was designed to detect the effects of adding water amount,decoction time times and optimize the extraction technology of the residues and other me-dicinal materials. Verification test was conducted. RESULTS:The optimal technology was L. japonica decocted first for 30 min with 8-fold water;the residues and other medicinal materials were decocted with 8-fold water for 3 times,1 h each time;combin-ing all the syrups. In verification test,the average contents of chlorohenic acid,linarin,harpagoside,(R,S)-epigoitrin,psoralen+angelicin were respectively 34.51,10.31,1.97,0.21,9.79 mg/g(RSD=1.24％,1.19％,1.40％,1.71％,1.28％,n=3);aver-age dry extraction yield was 25.4％(RSD=1.64％,n=3);average extraction rate of chlorohenic acid was 78.95％(RSD=1.24％,n=3). CONCLUSIONS:In the optimized water extraction technology,both the extraction rate of chlorohenic acid and contents of other ingredients are relatively high. The technology is stable and feasible.
ABSTRACT
Objective To study the protective effect of warming kidney and subsiding yang traditional Chinese medicine (TCM) method on lung tissues of rats with acute respiratory distress syndrome (ARDS) associated with sepsis. Methods Eighty healthy male Wistar rats were divided into normal control group, model group and low, medium and high dose Fusuheji groups by random number table, 16 rats in each group. The acute lung injury (ALI) model was established by injecting lipopolysaccharide (LPS) 3 mg/kg into a rat caudal vein within 5 minutes, and the normal control group was given the same volume of normal saline. Then the low, medium and high dose TCM groups were given low, medium and high dose Fusuheji TCM mixture (the ingredients of the mixture: radix aconite lateralis preparata 30 g, oysters 30 g, ginger 15 g, ephedra 15 g, licorice 10 g) 2.625, 7.875, 10.500 g/kg intragastric administration respectively. Equal volume of saline was given to the normal control group and model group by gavage. At 24 and 48 hours after respective administration, 8 rats were taken from each group to observe the pathological changes of lung tissues and score the lung injury. The rates of survival of rats were calculated after the experiment in various groups. Results After administration for 48 hours, the survival rate of rats in model group was obviously lower than that of the normal control group [18.7% (3/16) vs. 100.0% (16/16)], the low, middle and high dose Fusuheji groups' survival rates were all significantly higher than the rate of ALI model group [50.0% (8/16), 75.0% (12/16), 93.7%(15/16) vs. 18.7% (3/16), all P < 0.05]. There were no pathological changes in the lung tissues of rats in the normal control group, large amounts of exudates and hemorrhages were present in the lung tissues of ALI model group, and the inflammatory, exudative and hemorrhagic changes of lung tissues in the high, middle and low dose Fusuheji groups were obviously improved. After administration for 24 hours and 48 hours, the lung injury scores in the ALI model group were higher than those in control group (after administration for 24 hours: 7.83±0.60 vs. 2.89±4.23; after administration for 48 hours: 7.33±0.88 vs. 3.00±0.28), the scores of lung injury of any Fusuheji drug group were significantly lower than those of ALI model group, and the degrees of decrease were more marked in high dose Fusuheji group than those in low and middle dose Fusuheji groups (after administration for 24 hours: 3.37±0.32 vs. 6.00±0.44, 4.63±0.50; after administration for 48 hours: 3.25±0.25 vs. 5.25±0.25, 3.50±0.50). Conclusion The warming kidney and subsiding yang TCM method can improve the lung tissue injury in ARDS associated with sepsis in rats, promote the damaged lung tissue to recover, and ultimately the prognosis of ARDS rats is getting better.
ABSTRACT
Objective To study the protective effect of warming kidney and subsiding yang traditional Chinese medicine (TCM) method on lung tissues of rats with acute respiratory distress syndrome (ARDS) associated with sepsis. Methods Eighty healthy male Wistar rats were divided into normal control group, model group and low, medium and high dose Fusuheji groups by random number table, 16 rats in each group. The acute lung injury (ALI) model was established by injecting lipopolysaccharide (LPS) 3 mg/kg into a rat caudal vein within 5 minutes, and the normal control group was given the same volume of normal saline. Then the low, medium and high dose TCM groups were given low, medium and high dose Fusuheji TCM mixture (the ingredients of the mixture: radix aconite lateralis preparata 30 g, oysters 30 g, ginger 15 g, ephedra 15 g, licorice 10 g) 2.625, 7.875, 10.500 g/kg intragastric administration respectively. Equal volume of saline was given to the normal control group and model group by gavage. At 24 and 48 hours after respective administration, 8 rats were taken from each group to observe the pathological changes of lung tissues and score the lung injury. The rates of survival of rats were calculated after the experiment in various groups. Results After administration for 48 hours, the survival rate of rats in model group was obviously lower than that of the normal control group [18.7% (3/16) vs. 100.0% (16/16)], the low, middle and high dose Fusuheji groups' survival rates were all significantly higher than the rate of ALI model group [50.0% (8/16), 75.0% (12/16), 93.7%(15/16) vs. 18.7% (3/16), all P < 0.05]. There were no pathological changes in the lung tissues of rats in the normal control group, large amounts of exudates and hemorrhages were present in the lung tissues of ALI model group, and the inflammatory, exudative and hemorrhagic changes of lung tissues in the high, middle and low dose Fusuheji groups were obviously improved. After administration for 24 hours and 48 hours, the lung injury scores in the ALI model group were higher than those in control group (after administration for 24 hours: 7.83±0.60 vs. 2.89±4.23; after administration for 48 hours: 7.33±0.88 vs. 3.00±0.28), the scores of lung injury of any Fusuheji drug group were significantly lower than those of ALI model group, and the degrees of decrease were more marked in high dose Fusuheji group than those in low and middle dose Fusuheji groups (after administration for 24 hours: 3.37±0.32 vs. 6.00±0.44, 4.63±0.50; after administration for 48 hours: 3.25±0.25 vs. 5.25±0.25, 3.50±0.50). Conclusion The warming kidney and subsiding yang TCM method can improve the lung tissue injury in ARDS associated with sepsis in rats, promote the damaged lung tissue to recover, and ultimately the prognosis of ARDS rats is getting better.